bio rad c1000 touchtm thermal cycler (Bio-Rad)
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bio rad c1000 touchtm thermal cycler
Bio Rad C1000 Touchtm Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+c1000+touchtm+thermal+cycler/SsoAdvanced+Universal+SYBR+Green+Supermix/pm41986597-222-70-70
Average 99 stars, based on 8812 article reviews
Bio Rad C1000 Touchtm Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+c1000+touchtm+thermal+cycler/SsoAdvanced+Universal+SYBR+Green+Supermix/pm41986597-222-70-70
Average 99 stars, based on 8812 article reviews
bio rad c1000 touchtm thermal cycler - by Bioz Stars,
2026-09
99/100 stars
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Quantitative RT-PCR:Article Title: Stability of microRNAs in serum and plasma reveal promise as a circulating biomarker Article Snippet: All PCR reactions were performed in triplicate using iTaq Universal Probes Supermix (Bio-Rad, 1725131). .. RT-qPCR acquisition was performed using a Article Title: Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells. Article Snippet: RNA concentration was measured by Qubit 4 Fluorometer using the Qubit RNA HS Assay kit, and RNA purity was assessed by the Nanodrop 8000 Spectrophotometer. .. For each sample, 1 μg of total RNA was reverse-transcribed using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Catalog #1708841) following the manufacturer’s instructions. qPCR primers were designed for PAPR1 (forward primer 5’- TGGAAAAGTCCCACACTGGTA-3’; reverse primer 5’-AAGCTCAGAGAACCCATCCAC-3’) and the reference gene PGK1 (forward primer 5’- GACCTAATGTCCAAAGCTGAGAA-3’; reverse primer 5’- CAGCAGGTATGCCAGAAGCC-3’). qPCR reactions were set up in 10 μL volumes using SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Catalog #1725275) on a Article Title: Streptococcus pneumoniae upregulates Toll2, Toll9, and defensin genes in Bombyx larvae infection model. Article Snippet: The total RNA was extracted from the fat body in the dorsolateral region of larvae or tracheal bushes from all over the body using TRIzol® (Invitrogen, USA) as per the manufacturer’s guidelines. cDNA synthesis was performed with the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs) following the provided protocols. .. RT-qPCR was carried out using the Luna® Universal qPCR Master Mix (New England Biolabs) on a Article Title: Streptococcus pneumoniae upregulates Toll2 , Toll9 , and defensin genes in Bombyx larvae infection model Article Snippet: The total RNA was extracted from the fat body in the dorsolateral region of larvae or tracheal bushes from all over the body using TRIzol® (Invitrogen, USA) as per the manufacturer’s guidelines. cDNA synthesis was performed with the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs) following the provided protocols. .. RT-qPCR was carried out using the Luna® Universal qPCR Master Mix (New England Biolabs) on a Real-time Polymerase Chain Reaction:Article Title: Role of Long Non-Coding RNAs in Food Wanting of Apis Mellifera Article Snippet: Gene-specific primers were designed with Primer Premier 6.0 and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). ( ). .. The actin gene was used as an internal reference [ ]. qPCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) on a Article Title: Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells. Article Snippet: RNA concentration was measured by Qubit 4 Fluorometer using the Qubit RNA HS Assay kit, and RNA purity was assessed by the Nanodrop 8000 Spectrophotometer. .. For each sample, 1 μg of total RNA was reverse-transcribed using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Catalog #1708841) following the manufacturer’s instructions. qPCR primers were designed for PAPR1 (forward primer 5’- TGGAAAAGTCCCACACTGGTA-3’; reverse primer 5’-AAGCTCAGAGAACCCATCCAC-3’) and the reference gene PGK1 (forward primer 5’- GACCTAATGTCCAAAGCTGAGAA-3’; reverse primer 5’- CAGCAGGTATGCCAGAAGCC-3’). qPCR reactions were set up in 10 μL volumes using SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Catalog #1725275) on a Article Title: Streptococcus pneumoniae upregulates Toll2, Toll9, and defensin genes in Bombyx larvae infection model. Article Snippet: The total RNA was extracted from the fat body in the dorsolateral region of larvae or tracheal bushes from all over the body using TRIzol® (Invitrogen, USA) as per the manufacturer’s guidelines. cDNA synthesis was performed with the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs) following the provided protocols. .. RT-qPCR was carried out using the Luna® Universal qPCR Master Mix (New England Biolabs) on a Article Title: Streptococcus pneumoniae upregulates Toll2 , Toll9 , and defensin genes in Bombyx larvae infection model Article Snippet: The total RNA was extracted from the fat body in the dorsolateral region of larvae or tracheal bushes from all over the body using TRIzol® (Invitrogen, USA) as per the manufacturer’s guidelines. cDNA synthesis was performed with the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs) following the provided protocols. .. RT-qPCR was carried out using the Luna® Universal qPCR Master Mix (New England Biolabs) on a Reverse Transcription Polymerase Chain Reaction:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Reverse Transcription:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Article Title: Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells. Article Snippet: RNA concentration was measured by Qubit 4 Fluorometer using the Qubit RNA HS Assay kit, and RNA purity was assessed by the Nanodrop 8000 Spectrophotometer. .. For each sample, 1 μg of total RNA was reverse-transcribed using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Catalog #1708841) following the manufacturer’s instructions. qPCR primers were designed for PAPR1 (forward primer 5’- TGGAAAAGTCCCACACTGGTA-3’; reverse primer 5’-AAGCTCAGAGAACCCATCCAC-3’) and the reference gene PGK1 (forward primer 5’- GACCTAATGTCCAAAGCTGAGAA-3’; reverse primer 5’- CAGCAGGTATGCCAGAAGCC-3’). qPCR reactions were set up in 10 μL volumes using SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Catalog #1725275) on a Activation Assay:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Nested PCR:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Article Title: Unravelling of Leptospirosis in the Humid Subtropical Climate of the Eastern Part of Northern India Article Snippet: .. Amplification of the Leptospira-specific 16S rRNA gene (with primers Forward-A 5′-GGCGGCGCGTCTTAAACATG-3′, Reverse-B 5′-TTCCCCCCATTGAGCAAGATT-3′ and Nested PCR Forward-A 5′-TGCAAGTCAAGCGGAGTAGC-3′ and Reverse-B 5′-TTCTTAACTGCTGCCTCCCG-3′) was performed using conventional nested polymerase chain reaction (PCR) with modifications, utilizing the Positive Control:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Negative Control:Article Title: Long-term ecological surveillance of hard ticks (Acari: Ixodidae) and SFTSV in Dangjin, South Korea (2018-2024). Article Snippet: .. Primary RT-PCR was performed using a Amplification:Article Title: Molecular Characterization and Functional Insights into Goose IGF2BP2 During Skeletal Muscle Development Article Snippet: Each 20 uL reaction consisted of 10 uL of 2× TB Green Premix Ex Taq II (Tli RNaseH Plus) (Bio-Rad, Hercules, CA, USA), 2 μL of cDNA template, 0.5 μL of each primer (10 μM), and 7 μL of nuclease-free water. .. Amplification was performed on a Article Title: Unravelling of Leptospirosis in the Humid Subtropical Climate of the Eastern Part of Northern India Article Snippet: .. Amplification of the Leptospira-specific 16S rRNA gene (with primers Forward-A 5′-GGCGGCGCGTCTTAAACATG-3′, Reverse-B 5′-TTCCCCCCATTGAGCAAGATT-3′ and Nested PCR Forward-A 5′-TGCAAGTCAAGCGGAGTAGC-3′ and Reverse-B 5′-TTCTTAACTGCTGCCTCCCG-3′) was performed using conventional nested polymerase chain reaction (PCR) with modifications, utilizing the SYBR Green Assay:Article Title: Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells. Article Snippet: RNA concentration was measured by Qubit 4 Fluorometer using the Qubit RNA HS Assay kit, and RNA purity was assessed by the Nanodrop 8000 Spectrophotometer. .. For each sample, 1 μg of total RNA was reverse-transcribed using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Catalog #1708841) following the manufacturer’s instructions. qPCR primers were designed for PAPR1 (forward primer 5’- TGGAAAAGTCCCACACTGGTA-3’; reverse primer 5’-AAGCTCAGAGAACCCATCCAC-3’) and the reference gene PGK1 (forward primer 5’- GACCTAATGTCCAAAGCTGAGAA-3’; reverse primer 5’- CAGCAGGTATGCCAGAAGCC-3’). qPCR reactions were set up in 10 μL volumes using SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Catalog #1725275) on a Polymerase Chain Reaction:Article Title: Unravelling of Leptospirosis in the Humid Subtropical Climate of the Eastern Part of Northern India Article Snippet: .. Amplification of the Leptospira-specific 16S rRNA gene (with primers Forward-A 5′-GGCGGCGCGTCTTAAACATG-3′, Reverse-B 5′-TTCCCCCCATTGAGCAAGATT-3′ and Nested PCR Forward-A 5′-TGCAAGTCAAGCGGAGTAGC-3′ and Reverse-B 5′-TTCTTAACTGCTGCCTCCCG-3′) was performed using conventional nested polymerase chain reaction (PCR) with modifications, utilizing the |